Preclinical imaging technologies are powerful because they can propel science forward with fewer animals (consistent with the 3Rs), preserve tissues for additional assays, and empower translational studies. However, integrating imaging technologies into larger organizations is complex, requires deep knowledge of multiple fields, and a multidisciplinary team. Through this two-part series of webinars, we aim to educate on the strategic advantages that imaging technologies can bring to a scientific organization: how to select equipment, design facilities, and which key stakeholders to engage to make a successful imaging enterprise: and highlight the animal science required to conduct successful experiments.